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Yeasen Biotechnology fragmentation buffer
Fragmentation Buffer, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fragmentation+buffer/buffer+fragmentation/pmc12812582-67-15-17
Average 86 stars, based on 1 article reviews
fragmentation buffer - by Bioz Stars, 2026-09
86/100 stars

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Purification:

Article Title: Lobetyolin alleviates IMQ‑induced psoriasis‑like skin inflammation by maintaining the homeostasis of the skin and inhibiting the inflammatory cytokines in dendritic cells.
Article Snippet: RNA samples demonstrating high purity (A260/A280 ratio 1.8‐2.2) and RNA integrity numbers >7.0 were selected for subsequent library preparation using Illumina‐compatible protocols. mRNA was purified from 2 μg total RNA using mRNA Capture Beads 2.0 (cat. no. 12629ES; Shanghai Yeasen Biotechnology Co., Ltd.) through two rounds of poly(A) selection. .. Purified mRNA was fragmented in magnesium‐containing fragmentation buffer (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.) at 94 ̊C for 5 min. First‐strand cDNA was synthesized using random hexamer‐primed reverse tran‐ scription (SuperScriptTM IV Reverse Transcriptase; Thermo Fisher Scientific, Inc.). .. Second‐strand cDNA was generated using a dUTP incorporation strategy with E. coli DNA Polymerase I, RNase H, and dUTP solution (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.).

Article Title: Lobetyolin alleviates IMQ-induced psoriasis-like skin inflammation by maintaining the homeostasis of the skin and inhibiting the inflammatory cytokines in dendritic cells
Article Snippet: RNA samples demonstrating high purity (A260/A280 ratio 1.8-2.2) and RNA integrity numbers >7.0 were selected for subsequent library preparation using Illumina-compatible protocols. mRNA was purified from 2 μ g total RNA using mRNA Capture Beads 2.0 (cat. no. 12629ES; Shanghai Yeasen Biotechnology Co., Ltd.) through two rounds of poly(A) selection. .. Purified mRNA was fragmented in magnesium-containing fragmentation buffer (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.) at 94°C for 5 min. First-strand cDNA was synthesized using random hexamer-primed reverse transcription (SuperScriptTM IV Reverse Transcriptase; Thermo Fisher Scientific, Inc.). .. Second-strand cDNA was generated using a dUTP incorporation strategy with E. coli DNA Polymerase I, RNase H, and dUTP solution (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.).

Article Title: Cordycepin inhibits the proliferation and progression of NPC by targeting the MAPK/ERK and β-catenin pathways.
Article Snippet: .. The purified mRNA was cut into short fragments using fragmentation buffer (Shanghai Yeasen Biotechnology Co., Ltd.) and reverse transcribed into cDNA using random primers. ..

Article Title: Cordycepin inhibits the proliferation and progression of NPC by targeting the MAPK/ERK and β-catenin pathways
Article Snippet: .. The purified mRNA was cut into short fragments using fragmentation buffer (Shanghai Yeasen Biotechnology Co., Ltd.) and reverse transcribed into cDNA using random primers. ..

Synthesized:

Article Title: Lobetyolin alleviates IMQ‑induced psoriasis‑like skin inflammation by maintaining the homeostasis of the skin and inhibiting the inflammatory cytokines in dendritic cells.
Article Snippet: RNA samples demonstrating high purity (A260/A280 ratio 1.8‐2.2) and RNA integrity numbers >7.0 were selected for subsequent library preparation using Illumina‐compatible protocols. mRNA was purified from 2 μg total RNA using mRNA Capture Beads 2.0 (cat. no. 12629ES; Shanghai Yeasen Biotechnology Co., Ltd.) through two rounds of poly(A) selection. .. Purified mRNA was fragmented in magnesium‐containing fragmentation buffer (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.) at 94 ̊C for 5 min. First‐strand cDNA was synthesized using random hexamer‐primed reverse tran‐ scription (SuperScriptTM IV Reverse Transcriptase; Thermo Fisher Scientific, Inc.). .. Second‐strand cDNA was generated using a dUTP incorporation strategy with E. coli DNA Polymerase I, RNase H, and dUTP solution (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.).

Article Title: Lobetyolin alleviates IMQ-induced psoriasis-like skin inflammation by maintaining the homeostasis of the skin and inhibiting the inflammatory cytokines in dendritic cells
Article Snippet: RNA samples demonstrating high purity (A260/A280 ratio 1.8-2.2) and RNA integrity numbers >7.0 were selected for subsequent library preparation using Illumina-compatible protocols. mRNA was purified from 2 μ g total RNA using mRNA Capture Beads 2.0 (cat. no. 12629ES; Shanghai Yeasen Biotechnology Co., Ltd.) through two rounds of poly(A) selection. .. Purified mRNA was fragmented in magnesium-containing fragmentation buffer (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.) at 94°C for 5 min. First-strand cDNA was synthesized using random hexamer-primed reverse transcription (SuperScriptTM IV Reverse Transcriptase; Thermo Fisher Scientific, Inc.). .. Second-strand cDNA was generated using a dUTP incorporation strategy with E. coli DNA Polymerase I, RNase H, and dUTP solution (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.).

Reverse Transcription:

Article Title: Lobetyolin alleviates IMQ‑induced psoriasis‑like skin inflammation by maintaining the homeostasis of the skin and inhibiting the inflammatory cytokines in dendritic cells.
Article Snippet: RNA samples demonstrating high purity (A260/A280 ratio 1.8‐2.2) and RNA integrity numbers >7.0 were selected for subsequent library preparation using Illumina‐compatible protocols. mRNA was purified from 2 μg total RNA using mRNA Capture Beads 2.0 (cat. no. 12629ES; Shanghai Yeasen Biotechnology Co., Ltd.) through two rounds of poly(A) selection. .. Purified mRNA was fragmented in magnesium‐containing fragmentation buffer (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.) at 94 ̊C for 5 min. First‐strand cDNA was synthesized using random hexamer‐primed reverse tran‐ scription (SuperScriptTM IV Reverse Transcriptase; Thermo Fisher Scientific, Inc.). .. Second‐strand cDNA was generated using a dUTP incorporation strategy with E. coli DNA Polymerase I, RNase H, and dUTP solution (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.).

Article Title: Lobetyolin alleviates IMQ-induced psoriasis-like skin inflammation by maintaining the homeostasis of the skin and inhibiting the inflammatory cytokines in dendritic cells
Article Snippet: RNA samples demonstrating high purity (A260/A280 ratio 1.8-2.2) and RNA integrity numbers >7.0 were selected for subsequent library preparation using Illumina-compatible protocols. mRNA was purified from 2 μ g total RNA using mRNA Capture Beads 2.0 (cat. no. 12629ES; Shanghai Yeasen Biotechnology Co., Ltd.) through two rounds of poly(A) selection. .. Purified mRNA was fragmented in magnesium-containing fragmentation buffer (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.) at 94°C for 5 min. First-strand cDNA was synthesized using random hexamer-primed reverse transcription (SuperScriptTM IV Reverse Transcriptase; Thermo Fisher Scientific, Inc.). .. Second-strand cDNA was generated using a dUTP incorporation strategy with E. coli DNA Polymerase I, RNase H, and dUTP solution (cat. no. 12340ES97; Shanghai Yeasen Biotechnology Co., Ltd.).

Article Title: Cordycepin inhibits the proliferation and progression of NPC by targeting the MAPK/ERK and β-catenin pathways.
Article Snippet: .. The purified mRNA was cut into short fragments using fragmentation buffer (Shanghai Yeasen Biotechnology Co., Ltd.) and reverse transcribed into cDNA using random primers. ..

Article Title: Analysis of the molecular basis of Saccharomyces cerevisiae mutant with high nucleic acid content by comparative transcriptomics.
Article Snippet: Ribonucleic acid (RNA) and its degradation products are important functional components widely used in the food industry.. Transcription analysis was used to explore the genetic mechanism underlying nucleic acid synthesis in the chemical mutant Saccharomyces cerevisiae strain BY23-195 with high nucleic acid content.. Results showed that ribosome biogenesis, meiosis, RNA transport, mitogen-activated protein kinase (MAPK) signaling pathway, tryptophan metabolism, carbon metabolism, and longevity regulating pathway are closely related to the high nucleic acid metabolism of S. cerevisiae.

Article Title: Cordycepin inhibits the proliferation and progression of NPC by targeting the MAPK/ERK and β-catenin pathways
Article Snippet: .. The purified mRNA was cut into short fragments using fragmentation buffer (Shanghai Yeasen Biotechnology Co., Ltd.) and reverse transcribed into cDNA using random primers. ..

Isolation:

Article Title: Glutamate enhances the production of inflammatory cytokines IL-6 and IL-11, as well as chemokines CXCL2, CXCL3, and CXCL8 in keloid fibroblasts
Article Snippet: From 2 μg of total RNA, mRNA was enriched using mRNA Capture Beads 2.0 (Yeasen Biotechnology, Cat# 12629 ES, China) through two rounds of poly(A)-based selection. .. The isolated mRNA was then fragmented at 94 °C for 5 min in a magnesium-containing fragmentation buffer (Yeasen Biotechnology, Cat# 12340ES97). .. First-strand cDNA was synthesized by random hexamer-primed reverse transcription using SuperScriptTM IV Reverse Transcriptase (Thermo Fisher Scientific).

Magnetic Beads:

Article Title: Weighted Gene Co-Expression Network Analysis to Explore Hub Genes of Resveratrol Biosynthesis in Exocarp and Mesocarp of 'Summer Black' Grape.
Article Snippet: .. Eukaryotic mRNA was enriched with magnetic beads with Oligo (dT), and mRNA was randomly interrupted by the Fragmentation Buffer (YEASEN, Shanghai, China). ..

Article Title: Influence of cold-stimulated adipocyte supernatant on the expression of adhesion-related molecules in Schwann cell line
Article Snippet: .. The mRNA in each sample was enriched using oligo (dT) magnetic beads and fragmented with fragmentation buffer (approximately 200 bp). mRNA libraries were created using the Hieff NGSTM MaxUp Dual-mode mRNA Library Prep Kit for Illumina® (12301ES96, Yeasen Biotechnology (Shanghai) Co., Ltd., Shanghai, China) following the manufacturer's protocols. .. Libraries for paired-end sequencing were prepared (PE150; sequencing reads, 150 bp) at Sangon Biotech (Shanghai) Co., Ltd., using the MGISEQ-2000 platform.



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